ANIMAL BIOTECHNOLOGY

Paper Code: 
DZOL 611B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

DZOL 611B T

ANIMAL BIOTECHNOLOGY

The students will be able to-

 

CO1: Examine the basic concepts in Genetic Engineering and Cloning.

 

CO2: Deduce the use of Biotechnology in Medicine

 

CO 3:Describe the science of biotechnology and

biotechnology methods used to conduct experiments and develop products for

bioethical use.

 

 

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations,

Quiz

Assignments

Presentation

Individual and group projects

 

10.00

Introduction    

Basic Concept in Genetic Engineering

Enzymes used in Genetic Engineering: Restriction enzymes: Nomenclature, Types

DNA Ligase 

End Modification Enzymes

 
14.00

Molecular Techniques in Gene manipulation                  

Cloning vectors: Plasmids, Cosmids, Lambda Bacteriophage, M13, BAC, YAC, 

Transformation techniques: Calcium chloride method and electroporation.

Transduction

 
12.00

DNA libraries and sequencing techniques

Construction of genomic and cDNA libraries and screening by colony and plaque hybridization Southern, 

Northern and Western blotting 

DNA sequencing: Sanger method Polymerase Chain Reaction,

DNA Probes 

 
12.00

Genetically Modified Organisms                                

Production of cloned and transgenic animals: Nuclear Transplantation, Retroviral Method, DNA microinjection 

Applications of transgenic animals: Production of pharmaceuticals, production of donor organs, knock out mice

 
12.00

Culture Techniques and Applications                            

Animal cell culture, Expressing cloned genes in mammalian cells, 

Molecular diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia) 

Recombinant DNA in medicines: Recombinant insulin and human growth hormone,

Gene therapy 

 

Essential Readings: 

ESSENTIAL READINGS: 

  • Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.  

  • Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut. 

  • R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi. Principles of gene manipulation; An introduction to genetic engineering; Old, R.W and Primrose, S.B; Blackwell Science. 

  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi. 

  • Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006

  • Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company. 

 

SUGGESTED READINGS: 

  • Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.  

  • Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. 

  • Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA- Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y., USA. 

 

 e-RESOURCES: 

• NPTEL Biotechnology https://nptel.ac.in/courses/102103045

 • https://www.swayamprabha.gov.in/index.php/program/current_he/9 

• epg pathshala https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=t5vt4STquHRj94mcOBMr... • Immunology-ePGpathashala- Inflibnet centrehttps://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83...

e-Journal 

• https://nptel.ac.in/courses/102103045 

 
Academic Year: 
2023-2024
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