ANIMAL BIOTECHNOLOGY THEORY

Paper Code: 
25DZOL615B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Analyse the fundamental molecular tools and their applications
2. Appraise strategies of genetic engineering for possible applications in the biotechnology
3. Envisage the significance of genetic modifications or manipulations.
 
 
 
 
 
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

 

25DZOL615B

 

 

 

 

 

 

 

 

 

 

Animal Biotechnology

THEORY

CO:181 Examine the basic concepts in Genetic Engineering and enzymes involved.

CO:182Explain                                   various

Cloning Vectors, Transformation Techniques and Transduction

CO:183 Deduce various DNA libraries, blotting techniques, DNA probes and DNA sequencing methods

CO:184 Describe the science of biotechnology and biotechnology methods for construction                              transgenic animals

CO :185 Outline animal cell culture,                Expressing                cloned genes in mammalian cells, Molecular diagnosis of genetic diseases      and                    role       of Recombinant        DNA                   in medicines

CO:186 Contribute effectively in course-specific interaction

The           approach         in teaching: Interactive Lectures, Discussion, Tutorials,           Reading assignments, Demonstration,

Team teaching

Learning activities          for the students: Self-learning assignments,

Effective questions, Simulation

Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation.

 

12.00
Unit I: 
Introduction
Basic Concept in Genetic Engineering
Enzymes used in Genetic Engineering: Restriction enzymes: Nomenclature, Types DNA Ligase
End Modification Enzymes
12.00
Unit II: 
Molecular Techniques in Gene manipulation

Cloning vectors: Plasmids, Cosmids, Lambda Bacteriophage, M13, YAC, Transformation techniques: Calcium chloride method and electroporation

Transduction

12.00
Unit III: 
DNA libraries and sequencing techniques
Construction of genomic and cDNA libraries and screening by colony and plaque hybridization Southern, Northern and Western blotting
DNA sequencing: Sanger method, Polymerase Chain Reaction, DNA Probes
 
12.00
Unit IV: 
Genetically Modified Organisms

Production of cloned and transgenic animals: Nuclear Transplantation, Retroviral Method, DNA microinjection Applications of transgenic animals: Production of pharmaceuticals, production of donor organs, knock out mice.

12.00
Unit V: 
Culture Techniques and Applications
Animal cell culture, Expressing cloned genes in mammalian cells, Molecular diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)
Recombinant DNA in medicines: Recombinant insulin and human growth hormone, Gene therapy
 
Essential Readings: 
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.
● Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. Snustad, D.P. and Simmons, M.J. (2009). Principles of Genetics. V Edition, John Wiley and Sons Inc.
Essential Readings
● Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y.,USA.
● Beauchamp, T.I. and Childress, J.F. (2008). Principles of Biomedical Ethics. VI Edition, Oxford University Press. 
Academic Year: 
2025-2026
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