Coming soon
ANIMAL BIOTECHNOLOGY (Theory)
Course Objectives
This course will enable the students to –
1. Analyse the fundamental molecular tools and their applications
2. Appraise strategies of genetic engineering for possible applications in the biotechnology
3. Envisage the significance of genetic modifications or manipulations.
|
Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
|
Course Code |
Course Title |
|||
|
24DZOL601B |
Animal Biotechnology (Theory) |
CO97: Examine the basic concepts in Genetic Engineering and enzymes involved. CO98: Explain various Cloning Vectors, Transformation Techniques and Transduction CO99: Deduce various DNA libraries,blotting techniques, DNA probes and DNA sequencing methods CO100: Describe the science of biotechnology and biotechnology methods for construction transgenic animals CO101: Outline animal cell culture, Expressing cloned genes in mammalian cells, Molecular diagnosis of genetic diseases and role of Recombinant DNA in medicines CO102: Contribute effectively in course-specific interaction |
The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching
Learning activities for the students: Self-learning assignments, Effective questions, Simulation |
Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation. |
Enzymes used in Genetic Engineering: Restriction enzymes: Nomenclature, Types
DNA Ligase, End Modification Enzymes, Elementary Idea of cloning
Cloning vectors: Plasmids, Cosmids, Lambda Bacteriophage, M13, YAC
Transformation techniques: Calcium chloride method and electroporation.
Transduction
Construction of genomic and cDNA libraries and screening by colony and plaque hybridization
Southern, Northern and Western blotting
Polymerase Chain Reaction
DNA Probes
Production of cloned and transgenic animals: Nuclear Transplantation, Retroviral Method, DNA microinjection
Applications of transgenic animals: Production of pharmaceuticals, production of donor organs, knockout mice.
Human genome project
Hybridoma technology: Monoclonal antibodies in therapeutics and diagnosis
Animal cell culture: cell lines, primary and secondary cultures and culture media
Molecular diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)
Recombinant DNA in medicines: Recombinant insulin and human growth hormone
SUGGESTED READINGS
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.
●Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. Snustad, D.P. and Simmons, M.J. (2009). Principles of Genetics. V Edition, John Wiley and Sons Inc.
● Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y.,USA.
● Beauchamp, T.I. and Childress, J.F. (2008). Principles of Biomedical Ethics. VI Edition, Oxford University Press.
