ANIMAL BIOTECHNOLOGY PRACTICAL

Paper Code: 
25DZOL616B
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Gain knowledge on various instruments involved in biotechnology
2. Learn techniques of DNA isolation and blotting
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

25DZOL616

   (B)

 

 

 

 

 

 

 

 

 

 

Animal

Biotechnology

Practical

CO:187 Correlate the principles and working of various              instruments                 in biotechnology laboratory CO:188 Isolate    the genomic      and plasmid DNA

CO:189                     Conduct Restriction digestion CO:190        Gain        the knowledge     of blotting techniques and prepare report on Cell Culture CO:191 Demonstrate skills and record     the concepts in the form of practical file and reflect on         personal     learning experiences  and experimental           outcomes during viva voce.

CO:192 Contribute effectively in  course- specific interaction

The       approach             in teaching:

Interactive                          Lectures, Discussion,

Tutorials, Demonstration,

 

Learning activities for the students:

Self-learning assignments, Simulation, Chart preparation

Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation.

 

CONTENTS:
1. Genomic DNA isolation from E. coli
2. Plasmid DNA isolation (pUC 18/19) from E. coli
3. Restriction digestion of plasmid DNA.
4. Principle and working of equipment used in laboratory (Laminar Air Flow, Autoclave, Incubator, Electrophoresis chamber)
5. To study following techniques through photographs a. Southern Blotting b. Northern Blotting c. Western Blotting d. DNA Sequencing (Sanger's Method)
6. Project report on animal cell culture
 
SCHEME FOR PRACTICAL EXAMINATION:
 

Scheme for CA End Practical Examination

Exercise 1

Exercise II

File

Viva

Attendance

Total

5

5

10

5

5

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.
● Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. 
 
Academic Year: 
2025-2026
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