Coming soon
ANIMAL BIOTECHNOLOGY PRACTICAL
Course Objectives
This course will enable the students to –
1. Gain knowledge on various instruments involved in biotechnology
2. Learn techniques of DNA isolation and blotting
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Course Code |
Course Title |
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24DZOL602B |
ANIMAL BIOTECHNOLOGY PRACTICAL |
CO102: Correlate the principles and working of various instruments in biotechnology laboratory CO103: Isolate the genomic and plasmid DNA CO104 :Conduct Restriction digestion CO105: Gain the knowledge of blotting techniques and prepare report on Cell Culture CO106: Demonstrate skills and record the concepts in the form of practical file and reflect on personal learning experiences and experimental outcomes during viva voce.
CO107: Contribute effectively in course- specific interaction |
The approach in teaching: Interactive Lectures, Discussion, Demonstration, Team teaching Learning activities for the students: Self-learning assignments, Simulation, Chart preparation |
Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation. |
1. Plasmid DNA isolation (pUC 18/19) from E. coli
2. Restriction digestion of plasmid DNA.
3. Principle and working of equipment used in laboratory (Laminar Air Flow, Autoclave, Incubator, Electrophoresis chamber)
4. To study following techniques through photographs a. Southern Blotting b. Northern Blotting c. Western Blotting
5. Project report on animal cell culture
SCHEME FOR PRACTICAL EXAMINATION:
Scheme for CA End Practical Examination
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Exercise 1 |
Exercise II |
File |
Viva |
Attendance |
Total |
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5 |
5 |
10 |
5 |
5 |
30 |
Scheme for Semester End Practical Examination
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Exercise I |
Exercise II |
Exercise III |
Exercise IV |
Spotting |
File |
Viva |
Total |
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8 |
8 |
8 |
8 |
6x3=18 |
10 |
10 |
70 |
SUGGESTED READINGS
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.
● Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. Snustad, D.P. and Simmons, M.J. (2009). Principles of Genetics. V Edition, John Wiley and Sons Inc.
● Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y.,USA.
● Beauchamp, T.I. and Childress, J.F. (2008). Principles of Biomedical Ethics. VI Edition, Oxford University Press.
