ANIMAL BIOTECHNOLOGY PRACTICAL

Paper Code: 
24DZOL616B
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course Objectives
This course will enable the students to –
1. Gain knowledge on various instruments involved in biotechnology
2. Learn techniques of DNA isolation and blotting
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching strategies

Assessment

Strategies

 Course Code

Course Title

24DZOL 616(B)

Animal

Biotechnology

Practical

CO:187 Correlate the

principles and working of various instruments in biotechnology laboratory

CO:188 Isolate the genomic  and plasmid DNA

CO:189 Conduct Restriction  digestion

CO:190 Gain the knowledge  of blotting techniques and prepare report on Cell Culture

CO:191  Demonstrate skills and record the concepts in the form of  practical file and reflect on personal learning experiences and experimental outcomes during viva voce.

CO:192 Contribute effectively in course-specific interaction

The approach in  teaching:

Interactive Lectures,  Discussion, 

Tutorials,

Demonstration, 

 

Learning activities  for the students:

Self-learning 

assignments, 

Simulation, Chart

preparation

Assessment 

Strategies

Class test, 

Semester end 

examinations, 

Quiz, Solving 

problems in 

tutorials, 

Assignments, 

Presentation.

 

1. Genomic DNA isolation from E. coli 
2. Plasmid DNA isolation (pUC 18/19) from E. coli  
3. Restriction digestion of plasmid DNA. 
4. Principle and working of equipment used in laboratory (Laminar Air Flow, Autoclave,  Incubator, Electrophoresis chamber) 
5. To study following techniques through photographs a. Southern Blotting b. Northern  Blotting c. Western Blotting d. DNA Sequencing (Sanger's Method)  
6. Project report on animal cell culture 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA  End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

 

5

 

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 
 
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II  Edition, Academic Press, California, USA.  
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and  Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.  
● Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009).  An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA
 
Academic Year: 
2024-2025
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