ADVANCED BIOCHEMISTRY

Paper Code: 
25DZOL702
Credits: 
4
Contact Hours: 
4.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

1.   Enlighten the students about the importance of biological macromolecules, structures and configurational properties.

2. Examine the key role of biochemistry in the metabolic process.

Course Outcomes: 

Course

Learning outcomes (at course level)

Learning

and teaching strategies

Assessment Strategies

Course Code

Course Title

25DZO7

02

ADVANCED BIOCHEMISTR Y

CO114: Analyze the concepts of

biocatalysts, enzyme activity, its

kinetics,    inhibition               and  redox

reactions.

CO115:        Acquire                   detailed

knowledge about the structure,

classification,                        physico-chemical

properties        and                      biological

significance,                   metabolism                      and

metabolic                      disorders                       of

carbohydrates.

CO116:     Illustrate                 the                 lipid

classification,                      structure,

properties, anabolic and catabolic

pathways    of  lipid  metabolism

with    rate-limiting          steps          and disorders associated with them. CO117:   Classify proteins and

amino acids based on various

parameters.                       Compare                        and

contrast between different levels

of    protein       organization       and

correlate the protein sequencing

strategies with its structures.

CO118: Outline various catabolic

reactions associated with amino

acid degradation  and correlate

the inborn metabolic disorders

with  their  defective  metabolic

enzymes             and                         their

consequences.

CO119:Contribute  effectively in

course-specific interaction

Approach  in

teaching:

Interactive

Lectures,

Discussion,

Tutorials,

Reading

assignments,

Demonstration

,Team

teaching

Learning

activities for

the

students:

Self-learning assignments, Effective

questions,

Simulation,

Seminar

presentation,

Giving     tasks,

Field practical

Class test

Semester end

examinations,

Quiz,

Assignments,

Presentation

Individual

and       group

projects

 

12.00
Unit I: 
Enzymes

Biocatalysis

Classification and nomenclature of enzymes

Mechanism of enzyme action

Enzyme Kinetics

Inhibition of enzyme

Isoenzymes and coenzymes

Energy consideration

Biological oxidation and reduction

Fundamental reactions of biological oxidation

Redox potential and Electron transfer system enzymes

 

12.00
Unit II: 
Carbohydrates

Definition, general properties, classification and configuration of carbohydrates

Metabolism

Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle Phosphogluconate pathways (Pentose Phosphate Pathway), Glycogenesis, Glycogenolysis and Gluconeogenesis

Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.

12.00
Unit III: 
Lipids

Definition, general properties and classification Structure and importance of-:

Simple lipids- fats and wax

Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids Steroids- cholesterol, bile acids, steroid hormones, Lipoproteins

Lipid Metabolism

Beta oxidation and Lipogenesis.Inborn errors of metabolism: Niemann pick disease, TaySach’sdisease, Gaucher’s disease, Krabbe’s disease

12.00
Unit IV: 
Proteins

General properties and classification of proteins

Amino acid structure and classification

Structure of protein-Primary, Secondary, Tertiary And Quaternary

Ramachandran plots

Globular and fibrous protein- motif in globular proteins

Protein Sequencing

12.00
Unit V: 
Protein metabolism

Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea formation, Fate of carbon skeleton of amino acids (Metabolism of individual amino acid excluded)

Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism, Urea Disorders.

Essential Readings: 

ESSENTIAL READINGS:

●     U. Satyanaryana . Biochemistry IV Edition, Elsevier (2013)

●     Jain,J.L. Fundamentals of Biochemistry ,S.Chand publishers New Delhi.

●     Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.(2007)

●     Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman and Co.

●     Lehninger, A.D. Principles of Biochemistry, CBS Publishers and Distributors, Shahdara, Delhi.

●     Nelson, D. L., Cox, M. M. and Lehninger, A.L. (2009). Principles of Biochemistry. IV Edition. W.H. Freeman and Co

 

References: 

SUGGESTED READINGS:

●       Harvey, Richard A., Ph. D. (2011). Lippincott's illustrated reviews: Biochemistry.  Philadelphia

:Wolters Kluwer Health

●     Tortora, G.J. and Derrickson, B.H. (2009). Principles of Anatomy and Physiology, XII Edition, John Wiley & Sons, Inc.

●     Principles of Biochemistry; Smith et.al; McGraw Hill, New York.

●     Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G and Rajan, R; Orient Longman, Madras

●     Conn, E.E. and Stuff, P.K. Outlines of Biochemistry,John Wiley New York.

●     Donald Voet and Judith G. Voet Biochemistry, John Wiley and Sons., New York.

●     H.S. Srivastava, Element of Biochemistry, Rastogi Publications Meerut.

E-RESOURCES:

●       e-PGPATHSHALA -INFLIBNET CENTRE

●     https://epgp.inflibnet.ac.in/Home/ViewSubject?ca tid=MNhNzp1RQlU+6LM40KjY1Q==

●     Biochemistry NPTEL - https://onlinecourses.nptel.ac.in/noc22_cy06/preview

●     Biochemistry- Swayam Prabha-https://dth.ac.in/medical/course.php

e-JOURNAL:

●     Journal of Biochemistry- ISSN 1756-2651

●     Biochemistry- ACS Publication- ISSN 1520-4995

●     Journal of Cellular Biochemistry- Wiley online Library- Online ISSN: 1097-4644

 

Academic Year: 
2025-2026
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