ADVANCED BIOCHEMISTRY

Paper Code: 
24DZOL712
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to –
1. Enlighten the students about the importance of biological macromolecules, structures and configurational properties. 
2. Examine the key role of biochemistry in the metabolic process. 
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and  teaching

strategies

Assessment  Strategies

Course Code

Course Title

24DZOL

702

Advanced Biochemistry

THEORY

 

CO:199 Analyze the concepts  of biocatalysts, enzyme 

activity, its kinetics, inhibition and redox reactions.

CO:200 Acquire detailed 

knowledge about the 

structure, classification, 

physico-chemical properties  and biological significance, metabolism and metabolic disorders of  carbohydrates.

CO:201 Illustrate the lipid classification, structure, properties, anabolic  and catabolic pathways of lipid metabolism with  rate-limiting  steps and disorders associated with them.

CO:202 Classify proteins and amino acids based on various parameters. Compare and contrast between different levels of protein organization and  correlate the protein sequencing strategies with its structures.

CO:203 Outline various catabolic reactions associated with amino acid degradation and correlate the inborn metabolic disorders with their defective metabolic enzymes and their consequences.

CO:204 Contribute effectively in course-specific interaction

 

Approach in  teaching: Interactive

Lectures, Discussion, Tutorials, Reading assignments, Demonstration 

Learning activities for the students:

Self-learning assignments, Simulation, Seminar presentation, Giving tasks

Class test

Semester end  examinations, Quiz,

Assignments,  Presentation

Individual and group projects

 

12.00
Unit I: 
Enzymes
Biocatalysis
Classification and nomenclature of enzymes
Mechanism of enzyme action
Enzyme Kinetics
Inhibition of enzyme
Isoenzymes and coenzymes
Energy consideration
Biological oxidation and reduction
Fundamental reactions of biological oxidation
Redox potential and Electron transfer system enzymes
 
12.00
Unit II: 
Carbohydrates
Definition, general properties, classification and configuration of carbohydrates
Metabolism
Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle
Phosphogluconate pathways (Pentose Phosphate Pathway) Glycogenesis, Glycogenolysis  and Gluconeogenesis
Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage  diseases.
 
12.00
Unit III: 
Lipids
Definition, general properties and classification
Structure and importance of-:
Simple lipids- fats and wax
Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids 
Steroids- cholesterol, bile acids, steroid hormones Lipoproteins
Lipid Metabolism
Beta oxidation and Lipogenesis.Inborn errors of metabolism: Niemann pick disease,TaySach’sdisease,Gaucher’s disease,  Krabbe’s disease
 

 

12.00
Unit IV: 
Proteins
General properties and classification of proteins
Amino acid structure and classification
Structure of protein-Primary, Secondary, Tertiary And Quaternary
Ramachandran plots
Globular and fibrous protein- motif in globular proteins
Protein Sequencing
 
12.00
Unit V: 
Protein metabolism
Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination  reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea  formation, Fate of carbon skeleton of amino acids(Metabolism of individual amino acid  excluded)
Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism, Urea Disorders.
 
Essential Readings: 
ESSENTIAL READINGS:
● U. Satyanaryana . Biochemistry IV Edition, Elsevier (2013) 
● Jain,J.L. Fundamentals of Biochemistry ,S.Chand publishers New Delhi.
● Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.(2007)
● Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman and Co. 
● Lehninger, A.D. Principles of Biochemistry, CBS Publishers and Distributors, Shahdara,  Delhi.
● Nelson, D. L., Cox, M. M. and Lehninger, A.L. (2009). Principles of  Biochemistry. IV Edition. W.H. Freeman and Co
 
SUGGESTED READINGS:
● Harvey, Richard A., Ph. D. (2011). Lippincott's illustrated reviews: Biochemistry.  Philadelphia :Wolters Kluwer Health
● Tortora, G.J. and Derrickson, B.H. (2009). Principles of Anatomy and Physiology, XII  Edition, John Wiley & Sons, Inc. 
● Principles of Biochemistry; Smith et.al; McGraw Hill, New York. 
● Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G and Rajan, R; Orient  Longman, Madras 
● Conn, E.E. and Stuff, P.K. Outlines of Biochemistry,John Wiley New York. 
● Donald Voet and Judith G. Voet Biochemistry, John Wiley and Sons., New York.  
● H.S. Srivastava, Element of Biochemistry, Rastogi Publications Meerut. 
 
E-RESOURCES:
● e-PGPATHSHALA-INFLIBNET CENTRE
● Biochemistry- Swayam Prabha-https://dth.ac.in/medical/course.php
 
e-JOURNAL: 
● Journal of Biochemistry- ISSN 1756-2651
● Biochemistry- ACS Publication- ISSN 1520-4995
● Journal of Cellular Biochemistry- Wiley online Library- Online ISSN: 1097-4644
 
Academic Year: 
2024-2025
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