Practical

Paper Code: 
zol-215
Credits: 
4
Max. Marks: 
100.00
Objective: 

OBJECTIVES AND OUTCOMES

  • Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
  • Work collaboratively with other students and with faculty, understanding that most scientific knowledge on multiple sources of data and multiple experimental approaches.
  • Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
  • Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
  • Develop problem solving skills and analytical thinking skills.

 

 

Biochemistry II

  • Colorimetric/Spectrophotometric estimation of :
  • Carbohydrate-Glucose
  • Preparation of Phosphate buffer
  • Isolation of protein (casein) from milk
  • Study of temperature effect on enzyme action: Salivary amylase

 

Animal Diversity III

Study of museum specimens and slides:

 

Phylum  Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech

Dissection (Demonstration): Earthworm- Anatomy, Digestive, Nervous System

Permanent mount:Septalnephridia, Ovary,Nerve ring.

           

Phylum Arthropoda: Daphnia, Cyclops, Scorpion, King Crab, Crab, Eupagurus, Balanus, Millipede, Termite, Centipede, Praying-mantis, Peripatus

Dissection: (Demonstration): Prawn-General anatomy, Appendages, Nervous & Digestive system

Permanent mount: Hastate plate, Statocyst

 

Phylum-Mollusca:Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia

 

Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon

 

Larval forms- Miracidium, Sporocyst, Redia, Cercaria,Nauplius,Zoea,Mysis,cypris,Megalopa, Glochidium larva.

 

 

Molecular Biology:

 

  •  DNA isolation and separation by Agarose gel method
  • Vertical and horizontal electrophoresis unit (demonstration only)
  • Paper chromatographic separation of amino acids

 

Genetics &Biostatistics

  • Study of Drosophila life cycle & idea about its culture
  • Identification of Male & Female Drosophila
  • Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye, Apterus) of  Drosophila
  • Study of permanent prepared slide - Sex comb, Salivary gland chromosomes
  • Identification of Blood groups (A,B, O & Rh factor)
  • Preparation of frequency tables and graphs manually and with help of computers.
  • Calculation of Mean, Median, Mode.
  • Calculation of standard deviation, variance and standard error of the mean.
  • Chi square test for independence of attributes, t test

 

 

References: 

Suggested Readings:

  • A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K.Wilson& KH Goulding, ELBS Edition 1986.
  • Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.
  • Zubay G. Biochemistry, William C. Brown Pub.
  • Standard methods of biochemical analysis.Thimmaiah, S.R. KalyaniPublishers,Noida.
  • Pechenik, J.A. Biology of invertebrates,TataMc-Graw Hill Ltd, New Delhi.
  • Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
  • Practical Zoology, Invertebrates, Lal, S.S. Rastogi Publications, Meerut
  • Practical Zoology, Invertebrates ,Kohli,K.S., Ansari, A.S. Ramesh Book Depot, New Delhi.
  • Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book Depot, New Delhi.
  • Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K. Ramesh Book Depot, New Delhi.
  • Invertebrate Zoology Barnes, RD. W.B.Saunders Co., Philadelphia
  • Biostatistics; Gupta, S.P.;Sultan Chand & Co.
  • Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.

 

Academic Year: 
2019-2020
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