PRACTICAL

Paper Code: 
Z0L-225
Max. Marks: 
100.00
Objective: 

 

General physiology

 

  • Comparison of RBC and WBC in different groups of Vertebrates.
  • Photometric determination of haemoglobin in blood sample.
  • Determination of MCV,MCH,MCHC of the given sample of blood
  • Demonstration of the following in blood:clotting time,erythrocyte sedimentation rate,haemolysis and crenation
  • Determination of Haematocrit value of blood sample.

 

Tools and Techniques

 

  • Microscopes
  • Surgical techniques Adrenalectomy,Ovarectomy,Castration
  • Standardisation of oculometer and measurements of tubular diameter,cell heights.
  • To study the structure & function of Centrifuge.
  • To study the principle of UV & visible light spectroscopy.
  • To study the principle of cyclomixer.
  • Flow cytometry
  • To observe the sub cellular fractionation of rat liver.
  • Measurement of diameter of thyroid follicles.
  • Alizarin preparation.

 

Molecular Biology & Biotechnology

 

  • Chromosomal counting in bone marrow cells of a mammal
  • Isolation of RNA
  • Restriction  digestion of DNA
  • Agarose gel electrophoresis of DNA
  • Separation of amino acids on paper & thin layer chromatography
  • Separation of protein on SDS PAGE

 

 

Environmental Science and Wildlife Management

 

 

Water Evaluation Analysis( minimum of five analysis methods)

  • Determination of chloride the given water sample.
  • Estimation of total hardness of given water sample.
  • To determine the acidity of water.
  • To determine the alkalinity of water.
  • To estimate total solid in water.
  • To estimate total dissolved solid in water sample.
  • To determine the dissolved oxygen in given water sample.
  • To determine the BOD of given water sample.
  • To determine the free CO2 of given water sample.
  • To estimate the Calcium hardness of water.
  • To estimate Magnesium hardness of given water sample.

 

Soil Analysis( minimum of five analysis methods)

  • Determination of organic matter in soil sample.
  • Determination of Co3- & HCo3- in soil sample.
  • Estimation of chlorophyll in plant material.
  • Determination of leaf area index.
  • Determination of residual chloride in water sample.
  • Determination of moisture in soil.
  • Determination of minimum size of quadrant.
  • Determination of quadrate in field.
  • Study of community of quadrate.
  • Determination of dust holding capacity of leaves.
  • Determination of bulk density of the given soil sample.
  • Determination of water holding capacity of soil.
  • Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)