PRACTICAL

Paper Code: 
ZOL-225
Credits: 
9
Contact Hours: 
270.00
Max. Marks: 
100.00
Objective: 

OBJECTIVES AND OUTCOMES

  • Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
  • Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
  • Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
  • Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
  • Develop problem solving skills and analytical thinking skills.

 

 

General Physiology

 

  • Comparison of RBC and WBC in different groups of mammals.
  • Photometric determination ofhaemoglobin in blood sample.
  • Determination of MCV,MCH,MCHC of the given sample of blood
  • Demonstration of the following in blood:Clottingtime,Erythrocyte sedimentation rate,Haemolysis and Crenation
  • Determination of Haematocrit value of blood sample.

 

Tools and Techniques

 

  • Microscopes

 

  • Standardisation of Occulometer and measurements of tubular diameter,cell heights.
  • Measurement of diameter of thyroid follicles.
  • To study the structure & function of Centrifuge.
  • To study the principle of UV & visible light spectroscopy.
  • To study the principle of cyclomixer.
  • Flow cytometry
  • To observe the sub cellular fractionation of rat liver.
  • Alizarin preparation.

 

 

 

 

Molecular Biology & Biotechnology

 

  • Chromosomal counting in bone marrow cells of a mammal
  • Isolation of DNA
  • Restriction  digestion of DNA
  • Agarose gel electrophoresis of DNA
  • Separation of amino acids on paper &Thin layer Chromatography
  • Separation of protein on SDS PAGE

 

 

Environmental Science and Wildlife Management

 

 

 

Water Evaluation Analysis (minimum of five analysis methods)

  • Determination of chloride the given water sample.
  • Estimation of total hardness of given water sample.
  • To determine the acidity of water.
  • To determine the alkalinity of water.
  • To estimate total solid in water.
  • To estimate total dissolved solid in water sample.
  • To determine the dissolved oxygen in given water sample.
  • To determine the BOD of given water sample.
  • To determine the free CO2 of given water sample.
  • To estimate the Calcium hardness of water.
  • To estimate Magnesium hardness of given water sample.

 

Soil Analysis( minimum of five analysis methods)

  • Determination of organic carbon and organic matter in soil sample.
  • Determination of CO3-& HCO3- in soil sample.
  • Estimation of chlorophyll in plant material.
  • Determination of leaf area index and percent leaf injury of sample from polluted sites.
  • Determination of residual chloride in water sample.
  • Determination of moisture in soil.
  • Determination of frequency, density and abundance of community of quadrant
  • Determination of dust holding capacity of leaves.
  • Determination of bulk density of the given soil sample.
  • Determination of water holding capacity of soil.
  • Test for presence of inorganic salts in soil.

 

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)

 

Academic Year: 
2019-2020
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