Molecular Biology & Genetics II

Paper Code: 
ZOL-202
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to

1.     The course aims at studying the basic concepts of molecular biology and genetics

2.     To develop understanding of structure and packing of genetic material in the nucleus.

3.     How the DNA in a genome is organized, replicated, and repaired

4.     How genetic information in the DNA is selectively expressed as functional proteins.

5.     How genetic information transferred in the forms of codons and post translational modifications.

6.     Understanding of Chromosomes involved in Sex determination, chromosomal aberrations and Mutations.

7.     Learn human genetics and genetic counselling

Course Outcomes (COs): 

 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL202

Molecular Biology & Genetics II

The students will be able to –

CO19: Describe the Structure and packing of genetic material in the nucleus.

CO20: Describe the structure and types of Nucleic acids

CO21: Describe the process and importance of DNA replication, Transcription and translation in the cell.

CO22: Describe the chromosomes involved in sex determination

CO23: Understand how natural polymorphism and genetic variation can give rise to mutant genes, and how these genetic errors are inherited.      

 

Approach in teaching:

Class Lectures

Seminars, Discussion, Tutorials, Reading assignments, Demonstration,

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Group discussion

Class test, Semester end examinations

Quiz

Solving problems in tutorials

Assignments

Presentation

Individual and group projects

 

 

9.00
Unit I: 
UNIT I
Chromosomes: Morphology, Telomeres, Primary & secondary constrictions, Chromatids
Chromosome type- Polytene and Lampbrush
Chromosomal Organization: Euchromatin and heterochromatin 
Nucleosome concept

 

7.00
Unit II: 
UNIT II
DNA structure(A, B, Z type) 
Experiments of Messelson& Stahl 
Replication(Semi-conservative mechanism) of prokaryotes with reference to polymerases
Topoisomerases, Single stranded binding protein, Replication forks(Unidirectional & Bidirectional), Leading & lagging strand, RNA primers & Okazaki fragments

 

10.00
Unit III: 
UNIT III
RNA structure & types( mRNA, rRNA, tRNA)
Transcription of prokaryotes
(Idea about Polymerases)
Translation – Basic idea about  Protein synthesis  of prokaryotes
Genetic code

 

10.00
Unit IV: 
UNIT IV
Determination of Sex – Chromosomal mechanism & environmental influence, the sexual function of X &Y chromosome, Gynandromorphs, Genic Balance Theory
Sex linked inheritance in man (Colour-blindness &Haemophilia)& Drosophila (Eye colour), Y linked genes, Sex limited genes, Sex influenced genes

 

9.00
Unit V: 
UNIT V
Mutations- Chromosomal aberrations, Gene mutations
Human genetics- Chromosomal & single gene disorder, Genetics counseling, Amniocentesis
Eugenics & Euthenics
 
Essential Readings: 
Cell Biology by C.B. Powar; Himalaya Publishing House
A Textbook of Genetics; Rastogi, V.B.; Ramnath&Kedarnath.
Cell and Molecular Biology; Phillip Sheeler   Donald E. Bianchi; John Wiley and Sons. 
The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York.

 

References: 
Genetics; Winchester, A.M; Oxford and IBH Publishing Co. 
Genetics; Strickberger; Macmillan, Prentice Hall of India. 
Principles of Genetics; Gardener, E,J; Wiley eastern, New Delhi.
Primose, S.B. Tyman, R.M. and Old, R.W. Principles of gene manipulation, Willey-  Blacwell.
Cell and Molecular Biology; Gerald Karp; John Wiley and Sons,inc
Molecular Biology of the cell; Bruce Alberts, Julian Lewis, James D.Watson; Garland  Publishings
Cell and Molecular Biology; Phillip Sheeler,   Donald E. Bianchi; John Wiley and Sons.  Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the  Cell. VII Edition. Pearson Benjamin Cummings Publishing, San 
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, 
Peter Walter: Molecular Biology of the Cell, IV Edition.
Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition,  ASM Press and Sinauer Associates.
De Robertis, E.D.P. and De Robertis, E.M.F. (2006). Cell and Molecular Biology. VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition. John Wiley and Sons. Inc.
Lewin B. (2008). Gene XI, Jones and Bartlett
McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition. GS, Taylor and Francis Group, New York and London.
 

 

Academic Year: 
2020-2021
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