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Human genetics I (Practical) [1]

Paper Code: 
ZOL 324 D
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives
This course will enable the students to –
 
  1. Acquaint Hands on experience of Chromosomal preparation and karyotyping
  2. Explore to Culture Drosophila and identification of normal and mutant flies
  3. Appraise Experimental knowledge of DNA isolation, purification and quantification
  4. Describe about restriction endonucleases and electrophoresis
 
Course Outcomes (COs): 
 
 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL

324 D

 

Human genetics I

(Practical)

 

Students will –

CO111: Perform Chromosomal preparation and karyotyping

CO112:Assess to Culture Drosophila in laboratory and identify normal and mutant flies

CO113: Isolate, purify and quantify DNA, use of Restriction endonucleases and Electrophoresis.

CO 114: Explore EcoRI digestion of total DNA.

 

Approach in teaching:

Practical, Reading assignments, Demonstration

 

Learning activities for the students:

Practical  knowledge, Effective questions, Seminar presentation

 

Class test

Semester end examinations

Viva voce

 

 

CONTENTS
 
● Identification of normal and mutant flies ( Drosophila melanogaster) Preparation of Drosophila polytene Chromosomes (temporary and permanent preparations)
● Drosophila genetic crosses
● Chromosome preparation from mouse bone marrow and human blood lymphocytes G-banding
● Karyotyping
● Identification of inactivated X chromosome as Barr body and drumstick
● Genomic DNA isolation and purification from the nuclei and quantitation of DNA by spectrophotometer.
● EcoRI digestion of total DNA and separation of fragments by agarose gel Electrophoresis
 
 
Academic Year: 
2021-2022 [2]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/human-genetics-i-practical-0

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/human-genetics-i-practical-0
[2] https://zoology.iisuniv.ac.in/academic-year/2021-2022