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PRACTICAL [1]

Paper Code: 
ZOL-503
Credits: 
2
Max. Marks: 
100.00
Objective: 

OBJECTIVES AND OUTCOMES

  • Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
  • Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
  • Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
  • Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
  • Develop problem solving skills and analytical thinking skills.

 

Biotechnology and Microbiology:

 

  • Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)
  • Preparation and use culture media for microbes
  • Study of microbes in food material(curd, milk, rotten fruits and vegetables)
  • Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.)Candidates will have to submit a report on it.
  • Culturing of Bacteria from soil/water samples.

 

Immunology:

 

Experiments on Antigen and Antibody reaction.

  • Blood typing
  • ELISA
  • Ouchterlony double diffusion

 

 

Essential Readings: 

Suggested Reading:

  • Practical Biochemistry: Principles &Techniques; Keith Wilson& John Walker; Cambridge University Press.
  • Microbiology, A laboratory manual by Cappucino, James,G.
  • Lab Manual of microbiology & biotechnology by K. R. Aneja. Scientific international private limited.

 

 

 

 

Academic Year: 
2019-2020 [2]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/practical-29

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/practical-29
[2] https://zoology.iisuniv.ac.in/academic-year/2019-2020