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IMMUNOLOGY PRACTICAL [1]

Paper Code: 
25DZOL516A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Develop skills to determine antigen and antibody in the sample by various methods.
2. Perform blood typing assays and differentiate between blood cells.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

 

 

 

 

 

 

 

 

 

25DZOL516A

 

 

 

 

 

 

 

 

 

 

 Immunology        Practical

CO:127 Investigate the role of ELISA in antigen or antibody determination.

CO:128 Describe the importance of Ouchterlony double diffusion in qualitative determination of antigens

CO:129 Analyze the importance of agglutination reaction in blood groups determination and demonstrate different blood cells by preparing blood smear.

CO:130 Differentiate between histology of primary and secondary lymphoid organs

CO:131 Demonstrate skills and record the concepts in the form of practical file and reflect on personal learning experiences and experimental outcomes during viva voce

CO:132 Contribute effectively in course-specific interaction

The approach in teaching: Interactive Lectures, Reading assignments, Demonstration, Team teaching

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation,

Assessment Strategies Class                  test,

Semester                   end examinations, Solving problems        in tutorials, Assignments, Presentation.

 

CONTENTS:
1. Demonstration of lymphoid organs.
2. Histological study of spleen, thymus and lymph nodes through slides/ photographs
3. Preparation of stained blood film to study various types of blood cells.
4. Ouchterlony‘s double immuno-diffusion method.
5. ABO blood group determination.
6. Demonstration of Dot ELISA
* The experiments can be performed depending upon usage of animals in UG Courses.
 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA End Practical Examination

Exercise 1

Exercise II

File

Viva

Attendance

Total

5

5

10

5

5

30

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 
SUGGESTED READINGS
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology, VII Edition, Mosby, Elsevier Publication.
● Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.
Academic Year: 
2025-2026 [2]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/immunology-practical-3

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/immunology-practical-3
[2] https://zoology.iisuniv.ac.in/academic-year/2025-2026