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Molecular Biology [1]

Paper Code: 
25DZOL812
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to –
1. Develop an understanding of the life processes at the subcellular and molecular levels. 2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

CO:229 Develop an understanding

Approach in teaching:

Class test

 

 

of concepts and mechanisms of

Reading assignments,

Semester

 

 

replication

PowerPoint presentations

end

 

 

CO:230Appraise  a  Fundamental

Guest lecture

examinations

 

 

understanding of transcription and

Learning activities for

Quiz

 

 

post transcriptional modifications.

the students:

Assignments

 

 

25DZOL

802

Molecula r

Biology THEORY

CO:231 Develop an understanding of the translational and post translational process.

CO:232 Evaluate practical and theoretical  aspects  of  different

Simulation,

Seminar presentation and Self-learning assignments,

Presentation Model or chart preparation

 

 

molecular mapping techniques.

 

 

 

 

CO 233Compare and contrast

 

 

 

 

between different DNA

 

 

 

 

recombination and repair

 

 

 

 

techniques.

 

 

 

 

CO:234 Contribute effectively in

 

 

 

 

course-specific interaction

 

 

 

12.00
Unit I: 
DNA Replication
Prokaryotic & eukaryotic DNA replication
Mechanics of DNA replication
Enzymes and accessory proteins involved in DNA replication
 
12.00
Unit II: 
Transcription
Prokaryotic and Eukaryotic transcription
RNA polymerases
General and specific transcription factors
Post transcriptional modification in RNA
5’ cap formation
3’ end processing and polyadenylation
Splicing
Nuclear export of mRNA
mRNA editing 
 
12.00
Unit III: 
Translation
Genetic code
Prokaryotic and eukaryotic translation
Translational machinery
Mechanisms of Initiation, Elongation and termination
Regulation of Translation
Co and post translational modifications of protein
 
12.00
Unit IV: 
Molecular mapping
Molecular mapping of Genome
Genetic and physical maps
Southern and fluorescence in situ hybridization for genome analysis
Molecular markers in genome analysis: RFLP, RAPD and AFLP analysis
 
12.00
Unit V: 
Recombination and Repair
Homologous recombination: Holiday junction,
Site FLP/ FRT and Cre/lox recombination
Rec A and other Recombinases
DNA Repair mechanism : Mismatch repair, Excision repair, Direct repair, Recombination repair,
Double stranded break repair, SOS repair
 
Essential Readings: 
● Molecular Biology of the gene; Watson J.D.; Benzamin /Cummings.
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific AmericanBooks,USA,1994.
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press,2007.
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins,2005
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,1987
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc.,2005
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay
● Cell Biology by C.B. Powar; Himalaya Publishing House.
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.
 
SUGGESTED READING:
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
● Peter Walter: Molecular Biology of the Cell, IV Edition.
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
● Lewin B. (2008). Gene XI, Jones and Bartlett
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott Williams & Wilkins, 
2005
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
 
References: 
e CONTENT:
 
JOURNALS:
The Journal of Cell and Molecular Biology Cellular and Molecular Biology
Cellular and Molecular Life Sciences
Academic Year: 
2025-2026 [5]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/molecular-biology-10

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[1] https://zoology.iisuniv.ac.in/courses/subjects/molecular-biology-10
[2] https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA==
[3] https://nptel.ac.in/courses/104103121
[4] https://www.swayamprabha.gov.in/index.php/program/archive/9
[5] https://zoology.iisuniv.ac.in/academic-year/2025-2026