ADVANCED BIOCHEMISTRY

Published on Zoology (https://zoology.iisuniv.ac.in)

ADVANCED BIOCHEMISTRY [1]

Paper Code:

25DZOL702

Credits:

Contact Hours:

4.00

Max. Marks:

100.00

Objective:

Course objectives:

This course will enable the students to –

1. Enlighten the students about the importance of biological macromolecules, structures and configurational properties.

2. Examine the key role of biochemistry in the metabolic process.

Course Outcomes:

Course

Learning outcomes (at course level)

Learning

and teaching strategies

Assessment Strategies

Course Code

Course Title

25DZO7

ADVANCED BIOCHEMISTR Y

CO114: Analyze the concepts of

biocatalysts, enzyme activity, its

kinetics, inhibition and redox

reactions.

CO115: Acquire detailed

knowledge about the structure,

classification, physico-chemical

properties and biological

significance, metabolism and

metabolic disorders of

carbohydrates.

CO116: Illustrate the lipid

classification, structure,

properties, anabolic and catabolic

pathways of lipid metabolism

with rate-limiting steps and disorders associated with them. CO117: Classify proteins and

amino acids based on various

parameters. Compare and

contrast between different levels

of protein organization and

correlate the protein sequencing

strategies with its structures.

CO118: Outline various catabolic

reactions associated with amino

acid degradation and correlate

the inborn metabolic disorders

with their defective metabolic

enzymes and their

consequences.

CO119:Contribute effectively in

course-specific interaction

Approach in

teaching:

Interactive

Lectures,

Discussion,

Tutorials,

Reading

assignments,

Demonstration

,Team

teaching

Learning

activities for

the

students:

Self-learning assignments, Effective

questions,

Simulation,

Seminar

presentation,

Giving tasks,

Field practical

Class test

Semester end

examinations,

Quiz,

Assignments,

Presentation

Individual

and group

projects

12.00

Unit I:

Enzymes

Biocatalysis

Classification and nomenclature of enzymes

Mechanism of enzyme action

Enzyme Kinetics

Inhibition of enzyme

Isoenzymes and coenzymes

Energy consideration

Biological oxidation and reduction

Fundamental reactions of biological oxidation

Redox potential and Electron transfer system enzymes

12.00

Unit II:

Carbohydrates

Definition, general properties, classification and configuration of carbohydrates

Metabolism

Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle Phosphogluconate pathways (Pentose Phosphate Pathway), Glycogenesis, Glycogenolysis and Gluconeogenesis

Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.

12.00

Unit III:

Lipids

Definition, general properties and classification Structure and importance of-:

Simple lipids- fats and wax

Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids Steroids- cholesterol, bile acids, steroid hormones, Lipoproteins

Lipid Metabolism

Beta oxidation and Lipogenesis.Inborn errors of metabolism: Niemann pick disease, TaySach’sdisease, Gaucher’s disease, Krabbe’s disease

12.00

Unit IV:

Proteins

General properties and classification of proteins

Amino acid structure and classification

Structure of protein-Primary, Secondary, Tertiary And Quaternary

Ramachandran plots

Globular and fibrous protein- motif in globular proteins

Protein Sequencing

12.00

Unit V:

Protein metabolism

Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea formation, Fate of carbon skeleton of amino acids (Metabolism of individual amino acid excluded)

Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism, Urea Disorders.

Essential Readings:

ESSENTIAL READINGS:

● U. Satyanaryana . Biochemistry IV Edition, Elsevier (2013)

● Jain,J.L. Fundamentals of Biochemistry ,S.Chand publishers New Delhi.

● Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.(2007)

● Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman and Co.

● Lehninger, A.D. Principles of Biochemistry, CBS Publishers and Distributors, Shahdara, Delhi.

● Nelson, D. L., Cox, M. M. and Lehninger, A.L. (2009). Principles of Biochemistry. IV Edition. W.H. Freeman and Co

References:

ADVANCED BIOCHEMISTRY [1]

ESSENTIAL READINGS:

SUGGESTED READINGS:

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