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CELL BIOLOGY PRACTICAL [1]

Paper Code: 
24ZOL502(C)
Credits: 
2
Contact Hours: 
4.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

1. Construct knowledge about various fixation and staining techniques using different biological specimens.

2. Appraise their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.

Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

 

 

24DZOL502(C)

CELL BIOLOGY PRACTICAL

CO 79: Learn to prepare and

observe onion root tip squash

slides to identify mitotic stages.

CO 80: Study meiosis stages to

understand gamete formation.

CO  81:    Prepare  permanent

slides to visualize Barr bodies in

human female cells.

CO82:  Gain skills in fixation

and double and single staining

techniques using various fixatives and stains for cellular visualization

CO83: Demonstrate skills and

record the concepts in the form

of practical file and reflect on

personal learning experiences

and    experimental         outcomes

during viva voce.

CO84:Contribute effectively in

course-specific interaction

The approach

in teaching:

Interactive

Lectures,

Discussion,

Tutorials,

Reading

assignments,

Demonstration

, Team

teaching Learning activities for

the students:

Self-learning

assignments,

Effective

questions,

Simulation,

Seminar

presentation,

Giving tasks,

Chart

preparation

Assessment

Strategies

Class test,

Semester end

examinations,

Quiz, Solving

problems in

tutorials,

Assignments,

Presentation.

 

  1. Fixation and staining:
  2. Fixatives:- Carnoys, Bouins, formalin
  3. Stains:- Acetocarmine,Acetorcein, Eosin, Haematoxylin Double & Single staining :
  4. Staining of Euglena, Hydra, Obelia, Foraminiferous shell, Sponge spicules, Sponge gemmules,
  5. Spongin fibre
  6. Preparation of permanent slide to show the presence of Barr body in human female blood cells/cheek cells.
  7. Mitosis- Onion root tip (Temporary Slide preparation) and study of permanent slides Meiosis- Study of Permanent slides

 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA End Practical Examination

Exercise 1

Exercise II

File

Viva

Attendance

Total

 

5

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 

ESSENTIAL READINGS:

●     Practical Zoology, Vertebrates, Lal, S.S. Rastogi Publications, Meerut

●     Practical Zoology, Vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur.

●     Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.

References: 

SUGGESTED READINGS:

●     Thimmaiah, S.R. Kalyani Publishers, Noida

e-RESOURCES:

●     https://vlab.amrita.edu/?sub=3& [2] brch=64&sim=1090&cnt=2

●     Amrita Virtual Labs- http://biotech01.vlabs.ac.in/Lis t%20of%20experiments.html: [3]

 

Academic Year: 
2025-2026 [4]

Follow Zoology on:

IIS (Deemed to be University), Jaipur

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158

Source URL: https://zoology.iisuniv.ac.in/courses/subjects/cell-biology-practical-2

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/cell-biology-practical-2
[2] https://vlab.amrita.edu/?sub=3&
[3] http://biotech01.vlabs.ac.in/List%20of%20experiments.html
[4] https://zoology.iisuniv.ac.in/academic-year/2025-2026