Course objectives:
This course will enable the students to –
1. Develop skills to determine antigen and antibody in the sample by various methods.
2. Perform blood typing assays and differentiate between blood cells.
Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
|
Course Code |
Course Title |
|||
25DZOL5 02(A) |
IMMUNOLOGY PRACTICAL |
CO55: Investigate the role of ELISA in antigen or antibody determination. CO56: Describe the importance of Ouchterlony double diffusion in qualitative determination of antigens CO57: Analyze the importance of agglutination reaction in blood groups determination and prepare blood smear to analyse different blood cells CO58: Differentiate between histology of primary and secondary lymphoid organs CO59: Demonstrate skills and record the concepts in the form of practical file and reflect on personal learning experiences and experimental outcomes during viva voce. CO60: Contribute effectively in course-specific interaction |
The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Chart preparation |
Assessment Strategies Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation. |
1. Demonstration of lymphoid organs.
2. Histological study of spleen, thymus and lymph nodes through slides/ photographs
3. Preparation of stained blood film to study various types of blood cells.
4. Ouchterlony’s double immuno-diffusion method.
5. ABO blood group determination.
6. Demonstration of Dot ELISA
Scheme for CA End Practical Examinat ion
Exercise 1 |
Exercise II |
File |
Viva |
Attendance |
Total |
5 |
5 |
10 |
5 |
5 |
30 |
Scheme for Semester End Practical Examinat ion
Exercise I |
Exercise II |
Exercise III |
Exercise IV |
Spotting |
File |
Viva |
Total |
8 |
8 |
8 |
8 |
6x3=18 |
10 |
10 |
70 |
● Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006 ∙
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.
* The experiments can be performed depending upon usage of animals in UG Courses.
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology, VII Edition, Mosby, Elsevier
Publication.
● Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.
e-CONTENT:
● Immunology- ePG pathshala- Inflibnet centre-
● https://epgp.inflibnet.ac.in/Home/ViewSubject?ca [2] tid=2rAs1Puvga4LW93zMe83aA == ∙ Immunology-
NPTEL- https://nptel.ac.in/courses/102103038 [3]
e-JOURNAL:
● Immunology- Wiley online Library- ISSN 1365-2567
● Scandinavian Journal of Immunology-Online ISSN: 1365-3083
Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/immunology-practical-2
[2] https://epgp.inflibnet.ac.in/Home/ViewSubject?ca
[3] https://nptel.ac.in/courses/102103038
[4] https://zoology.iisuniv.ac.in/academic-year/2025-2026