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IMMUNOLOGY PRACTICAL [1]

Paper Code: 
25DZOL502(A)
Credits: 
4
Contact Hours: 
4.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

1.  Develop skills to determine antigen and antibody in the sample by various methods.

2.   Perform blood typing assays and differentiate between blood cells.

Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25DZOL5

02(A)

IMMUNOLOGY

PRACTICAL

CO55: Investigate the role of

ELISA in antigen or antibody

determination.

CO56:         Describe                   the

importance     of                    Ouchterlony

double diffusion in qualitative

determination of antigens

CO57:          Analyze                    the

importance    of  agglutination

reaction    in    blood                groups

determination     and                        prepare

blood     smear     to             analyse

different  blood cells

CO58:

Differentiate                                between

histology    of    primary                 and

secondary lymphoid organs

CO59: Demonstrate skills and

record the concepts  in the

form  of     practical    file  and

reflect  on  personal  learning

experiences and experimental

outcomes during viva voce.

CO60: Contribute effectively

in course-specific interaction

The approach in

teaching:

Interactive

Lectures,

Discussion,

Tutorials, Reading

assignments,

Demonstration,

Team teaching

Learning activities

for the students:

Self-learning

assignments,

Effective questions,

Simulation,

Seminar

presentation,

Giving tasks, Chart

preparation

Assessment

Strategies

Class             test,

Semester end

examinations,

Quiz, Solving

problems                 in

tutorials,

Assignments,

Presentation.

 

CONTENTS:

1. Demonstration of lymphoid organs.

2. Histological study of spleen, thymus and lymph nodes through slides/ photographs

3. Preparation of stained blood film to study various types of blood cells.

4. Ouchterlony’s double immuno-diffusion method.

5. ABO blood group determination.

6. Demonstration of Dot ELISA

 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA End Practical Examinat ion

Exercise 1

Exercise II

File

Viva

Attendance

Total

 

5

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examinat ion

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 

ESSENTIAL READINGS:

●     Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006 ∙

●     Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.

* The experiments can be performed depending upon usage of animals in UG Courses.

References: 

SUGGESTED READINGS:

●     Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.

●     David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology, VII Edition, Mosby, Elsevier

Publication.

●     Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.

e-CONTENT:

●     Immunology- ePG pathshala- Inflibnet centre-

●     https://epgp.inflibnet.ac.in/Home/ViewSubject?ca [2] tid=2rAs1Puvga4LW93zMe83aA ==  ∙ Immunology-

NPTEL- https://nptel.ac.in/courses/102103038 [3]

e-JOURNAL:

●     Immunology- Wiley online Library- ISSN 1365-2567

●     Scandinavian Journal of Immunology-Online ISSN: 1365-3083

 

Academic Year: 
2025-2026 [4]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/immunology-practical-2

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/immunology-practical-2
[2] https://epgp.inflibnet.ac.in/Home/ViewSubject?ca
[3] https://nptel.ac.in/courses/102103038
[4] https://zoology.iisuniv.ac.in/academic-year/2025-2026