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MOLECULAR BIOLOGY [1]

Paper Code: 
24DZOL812
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:  
This course will enable the students to – 
1. Develop an understanding of the life processes at the subcellular and molecular levels.  2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries. 
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and teaching 

strategies

Assessment  Strategies

Course

Code

Course Title

24DZOL 802

Molecular

Biology

THEORY

 

CO:229 Develop an understanding of concepts and mechanisms of replication

CO:230 Appraise a Fundamental understanding of transcription and post transcriptional modifications.

CO:231 Develop an understanding of the translational and post translational process.

CO:232 Evaluate practical and theoretical aspects of different molecular mapping techniques.

CO 233 Compare and contrast between different DNA recombination and repair techniques.

CO:234 Contribute effectively in course-specific interaction

Approach in teaching: Reading assignments, 

PowerPoint presentations

Guest lecture

Learning activities for 

the students: Simulation, 

Seminar presentation and  Self-learning assignments, 

 

Class test 

Semester 

end 

examinations Quiz

Assignments  Presentation Model or 

chart 

preparation

 

12.00
Unit I: 
DNA Replication
Prokaryotic & eukaryotic DNA replication 
Mechanics of DNA replication 
Enzymes and accessory proteins involved in DNA replication 
 
12.00
Unit II: 
Transcription
Prokaryotic and Eukaryotic transcription 
 RNA polymerases 
General and specific transcription factors 
Post transcriptional modification in RNA 
5’ cap formation 
3’ end processing and polyadenylation 
Splicing 
Nuclear export of mRNA 
mRNA editing 
 
12.00
Unit III: 
Translation
Genetic code 
Prokaryotic and eukaryotic translation 
 Translational machinery 
 Mechanisms of Initiation, Elongation and termination 
 Regulation of Translation 
 Co and post translational modifications of protein 
 
12.00
Unit IV: 
Molecular mapping
Molecular mapping of Genome 
Genetic and physical maps 
Southern and fluorescence in situ hybridization for genome analysis 
Molecular markers in genome analysis: RFLP, RAPD and AFLP analysis 
 
12.00
Unit V: 
Recombination and Repair
Homologous recombination: Holiday junction, 
Site FLP/ FRT and Cre/lox recombination 
Rec A and other Recombinases 
DNA Repair mechanism : Mismatch repair, Excision repair, Direct repair, Recombination repair, 
Double stranded break repair, SOS repair
 
Essential Readings: 
ESSENTIAL READING: 
● Molecular Biology of the gene; Watson J.D.; Benzamin /Cummings. 
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K.  Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994
● Molecular Cell  biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific  AmericanBooks,USA,1994. 
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM  Press,2007. 
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott  Williams & Wilkins,2005 
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd.,  1990 
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons,  Inc,1987 
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley  & sons, Inc.,2005 
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay 
● Cell Biology by C.B. Powar; Himalaya Publishing House.  
● Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.  
 
SUGGESTED READING: 
● The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York 
● Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the  Cell.VII Edition. Pearson Benjamin Cummings Publishing, San  
● Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,  
● Peter Walter: Molecular Biology of the Cell, IV Edition.  
● Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition,  ASM Press and Sinauer Associates.  
● De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII  Edition. Lippincott Williams and Wilkins, Philadelphia.  
● Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John  Wiley and Sons.Inc.  
● Lewin B. (2008). Gene XI, Jones and Bartlett  
● McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition.GS,  Taylor and Francis Group, New York and London.  
● Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings. 
● Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific  American Books,USA,1994.  
● Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties, Lippincott  Williams & Wilkins, 2005  
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons,  Inc, 1987  
 
 
e CONTENT: 
 
JOURNALS: 
The Journal of Cell and Molecular Biology 
Cellular and Molecular Biology  
Cellular and Molecular Life Sciences
 
Academic Year: 
2024-2025 [5]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/molecular-biology-8

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/molecular-biology-8
[2] https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83aA== 
[3] https://nptel.ac.in/courses/104103121 
[4] https://www.swayamprabha.gov.in/index.php/program/archive/9 
[5] https://zoology.iisuniv.ac.in/academic-year/2024-2025