Practical

Practical [1]

Paper Code:

ZOL 225

Credits:

Contact Hours:

270.00

Max. Marks:

100.00

Objective:

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 225

Practical

Students will –

CO53: Relate and describe different laboratory specimens based on their external features and appearance and their categorical relation with various phyla.

CO54: Appraise hands-on experience of various molecular biology techniques including gel electrophoresis and chromatography and would learn about their applications in the research field.

CO55: Appraise hands-on practice of various microscopic techniques, occulometery, centrifugation and UV - visible spectroscopy.

CO56: Differentiate and evaluate various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO57:Evaluate practical and theoretical aspects of different molecular techniques, physiological and environmental techniques

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the students:

Self learning assignments, Effective questions, Simulation, Giving tasks, Field practical

Class test

Semester end examinations

Viva voce

Presentation

General Physiology

● Comparison of RBC and WBC in different groups of mammals.

● Photometric determination of hemoglobin in blood sample.

● Determination of MCV,MCH,MCHC of the given sample of blood

● Demonstration of the following in blood:Clotting time,Erythrocyte sedimentation rate,Haemolysis andCrenation

● Determination of Haematocrit value of blood sample.

Tools and Techniques

● Microscopes

● Standardization of Occulometer and measurements of tubular diameter,cell heights.

● Measurement of diameter of thyroid follicles.

● To study the structure & function of Centrifuge.

● To study the principle of UV & visible light spectroscopy.

● To study the principle of cyclomixer.

● Flow cytometry

● To observe the subcellular fractionation of rat liver.

● Alizarin preparation.

Molecular Biology & Biotechnology

● Chromosomal counting in bone marrow cells of a mammal

● Isolation of DNA

● Restriction digestion of DNA

● Agarose gel electrophoresis ofDNA

● Separation of amino acids on paper andThin layerChromatography

● Separation of protein on SDS PAGE

Environmental Science and Wildlife Management

Water Analysis (minimum of five analysis methods)

● Determination of chloride in the given water sample.

● Estimation of total hardness of given water sample.

● To determine the acidity of water.

● To determine the alkalinity of water.

● To estimate total solid in water.

● To estimate the total dissolved solid in the water sample.

● To determine the dissolved oxygen in a given water sample.

● To determine the BOD of a given water sample.

● To determine the free CO2 of a given water sample.

● To estimate the Calcium hardness of water.

● To estimate Magnesium hardness of a given water sample.

Soil Analysis (minimum of five analysis methods)

● Determination of organic carbon and organic matter in soil sample.

● Determination of CO3-& HCO3- in soil sample.

● Estimation of chlorophyll in plant material.

● Determination of leaf area index and percent leaf injury of sample from polluted sites.

● Determination of residual chloride in water sample.

● Determination of moisture in soil.

● Determination of frequency, density and abundance of community of quadrant

● Determination of dust holding capacity of leaves.

● Determination of bulk density of the given soil sample.

● Determination of water holding capacity of soil.

● Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report

Essential Readings:

ESSENTIAL READINGS:

Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, (6^th Edition),2007.
Handbook of methods in Environmental Studies,Vol 2 (Air, noise, soil and overburden analysis) Maiti,S.K., Jaipur Publications.
Handbook of Methods in Environmental Studies, Vol I, Water and Wastewater, Maiti,S.K., Jaipur Publications.
Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc.,1998.

SUGGESTED READING:

• Sembulingam, K.Sembulingam. Essentials of medical Physiology, Jaypee Brothers Medical Publishers, New Delhi.

• TextBook of Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.( 2007)

• Principles of Wildlife management; James, A. Bailey; John Wiley & sons

• Introduction to Instrumental analysis; Robert Braun: Mc –Graw hill, International eds.

• Environmental Science, Santra S.C., New Central Book Agency Pvt Ltd., Kolkata

• Environmental Science, Cunningham andSaigo

e-RESOURCES:

• e-PGpathshala- inflibnet center-

https://epgp.inflibnet.ac.in/Home/ViewSubject?catid=2rAs1Puvga4LW93zMe83... [2]

● https://esj-JOURNALS:.onlinelibrary.wiley.com/journal/1438390x [3]

● https://bioprinciples.biosci.gatech.edu/population-ecology-1/ [4]