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PRACTICAL [1]

Paper Code: 
ZOL 125
Credits: 
9
Contact Hours: 
30.00
Max. Marks: 
100.00
Objective: 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 125

Practical

The students will –

 

CO22: Illustrate different laboratory specimens based on their external features and appearance and their categorical relation with various phylum.

CO23: Students will get hands-on experience of various fixation and staining techniques using different biological specimens.

CO24: Enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.

CO25: Experiment various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO26: Analyse the effect of temperature on the enzymatic activity and gain hands-on expertise in separation of various solutes using chromatographic techniques.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

 

Class test

Semester end examinations

Viva voce

Assignments

Presentation

Individual and group projects

 

Invertebrates

Identification, classification & study of distinguishing features of representatives from various groups.

  • Phylum Protozoa- Polystomella, Formanifera, Opalina, Paramecium(Fission & conjugation) Vorticella, Euglena,Trypanosoma,Monocystis,Plasmodium.
  • Phylum Porifera-Sycon L.S & T.S, Spicules, Spongin fibres, Leucosolenia, Euplectella
  • Phylum Cnidaria- Obelia,(Polyp & Medusa), Millepora, Physalia,Pennatula, Metridium, Madrepora, Alcyonium, Gorgonia, Aurelia.
  • Phylum Helminthes- Ascaris, Taenia,Planaria
  • Phylum Annelida- Pontobdella, Aphrodite, Leech, Polygordius,Chaetopterus, Nereis, Heteronereis,Arenicola.
  • Phylum Arthropoda- Cyclops, Peripatus, Balanus, Lepas, Hippa, Belostoma, Limulus, Eupagurus, Julus, Scolopendra, Prayingmantis.
  • Phylum Mollusca- Murex, Bulla, Cardium, Arca, Turritella, Pinctada,Cypraea, Octopus,Nautilus.
  • Phylum Echinodermata- Echinus, Holothuria, Antedon, Asterias
  • Minor Phyla- Bugula, Plumatella, Crestatella, Pectinella,Phoronis,Dendrostoma
  • Larval Stages: Planula, Redia, Miracidium, Sporocyst,Cercaria,Metacercaria, Trochophore, Nauplius, Zoea, Mysis, Phyllosoma, Trilobite larva ofLimulus, Glochidium, Bipinnaria, Echinopluteus, Auricularia, Tornaria

Dissections:

  • Cockroach: General anatomy, Reproductive system, Nervous system Mouthparts:Cockroach and Mosquito

Biosystematics, taxonomy and evolution

  • Composition and assessment of the taxonomic diversity/biodiversity in a habitat (e.g. grassland, arid land, wet land etc.)
  • Estimation of gene and genotypic frequencies in light of Hardy-Weinberg law based on facial traits/thumb prints, ABO blood group data etc.
  • Project: Preparation of models showing the status of certain taxa or species in a particular habitat.
  • Identification of Phytoplanktons and Zooplanktons from fresh water samples.

Cell Biology

  • Preparation of different cell types: Liver Hepatocytes , Adipocytes, Spleen cells
  • Study of Polytene chromosomes in salivary glands ofChironomus larva/Drosophila
  • Light microscopic examination and preparation of tissue sections.
  • Study of mitotic and meiotic chromosomes from permanent prepared slides.
  • Preparation of slides for mitotic study.
  • Preparation of slides for meiotic study in the testis of grasshopper.
  • Preparation of multipolar nerve cells from the spinal cord of mammal.
  • Barr body preparation in buccal epithelial cells.

Biochemistry

  • Qualitative analysis of Lipid, protein and Carbohydrate samples
  • Colorimetric/Spectrophotometric estimation of:
    • Carbohydrate- glycogen,
    • cholesterol
    • Protein
    • Nucleic acids:DNA,RNA
  • Study of temperature effect on enzyme action: salivary amylase, liver catalase

 

Essential Readings: 
ESSENTIAL READINGS: 
Vertebrates Comparative anatomy, function, evolution, K.V. Kardong, Tata-McGraw  Hill,New Delhi. 
● Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut 
● Practical Zoology, vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur. 
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.
● Wilson,K. and Walker, J. Principles and techniques of practical biochemistry,  Cambridge press.
● Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
● Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.
 
REFERENCES: 
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub. 
● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida
 
E-RESOURCES: 
● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102 [2]
● Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64  [3]
● Biotechnology and Biomedical Engineering- NPTEL labs- https://www.vlab.co.in/ba-nptel-labs-biotechnology-and-biomedical-engine... [6]
 

 

Academic Year: 
2023-2024 [7]

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Source URL: https://zoology.iisuniv.ac.in/courses/subjects/practical-131

Links:
[1] https://zoology.iisuniv.ac.in/courses/subjects/practical-131
[2] https://nptel.ac.in/courses/104105102
[3] https://vlab.amrita.edu/?sub=3&brch=64%C2%A0
[4] https://vlab.amrita.edu/?sub=3&brch=64&sim=1090&cnt=2
[5] http://biotech01.vlabs.ac.in/List%20of%20experiments.html:
[6] https://www.vlab.co.in/ba-nptel-labs-biotechnology-and-biomedical-engineering
[7] https://zoology.iisuniv.ac.in/academic-year/2023-2024